Infected leaves were cut into small pieces (5mm infected tissue and 5mm of adjacent healthy tissue) and placed in 70 % ethanol for 10 seconds, washed twice with autoclaved distilled water and dipped in 300ml autoclaved distilled water for 15 minutes in microcentrifuge tube. Leaf pieces were then placed and bacterial ooze in water was streaked on Nutrient Agar (NA) medium (Nutrient broth 2.5%, Agar 2%) plates and left in incubator at 30°C overnight for the isolation of Xoo