The study was carried out at the Department of Botany, Aligarh Muslim University, India. During the investigation, ornamental plants belonging to family Asteraceae (Calendula officinalis, Centaurea montana, Chrysanthemum morifolium), Caryophyllaceae (Dianthus caryophyllus), and Papaveraceae (Papaver somniferum)as the test plants and root-knot nematode M. incognita as a test pathogen were selected